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BacteriorhodopsinExpression, Purification, and Structural Characterization of the Bacteriorphodopsin
Aspartyl Transcarbamylase Fusion Protein. Two-dimensional
crystallization of Escherichia coli-expressed bacteriorhodopsin
and its D96N variant: High resolution structural studies in projection. Bacteriorhodopsin D85N: Three
spectroscopic species in equilibrium. Wild-type and mutant bacteriorhodopsins
D85N, D96N, and R82Q: purification
to homogeneity, pH dependence of pumping, and electron diffraction. Wild-type and mutant bacterioopsins D85N,
D96N, and R82Q: high-level expression
in Escherichia coli. Effects of Asp-96—Asn, Asp-85—Asn,
and Arg-82—Gln single-site
substitutions on the photocycle of bacteriorhodopsin. Resonance raman spectra of bacteriorhodopsin
mutants with substitutions at Asp-85, Asp-96, and Arg-82. Effects of detergent
environments on the photocycle of purified monomeric
bacteriorhodopsin. High sensitivity electron diffraction analysis. A study of divalent cation
binding to purple membrane. Preparative purification of functional
bacteriorhodopsin by high-performance size-exclusion chromatography. Purification of bacteriorhodopsin
and characterization of mature and partially processed forms. Cation binding
sites on the projected structure of bacteriorhodopsin. Location of an extrinsic
label in the primary and tertiary structure of bacteriorhodopsin. The structure of acetylcholine
receptor and of bacteriorhodopsin. Linking regions between helices in bacteriorhodopsin revealed. A probable linking sequence between
two transmembrane components of bacteriorhodopsin. Projected structure of purple membrane
determined to 3.7Å resolution
by low temperature electron microscopy. Attachment site(s) of
retinal in bacteriorhodopsin. |
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