Agard Laboratory General Behavior

1. Remember you are not the only one in the lab. Leave all common areas cleaner than you found them. Treat all equipment gently. Do not yank or pull things by their cords or electrical leads. Always pay attention to what your doing. Clean-up all spills, droppings or breakage. Remember to turn-off equipment when finished.

2. If you break something or find it broken, do not leave it for someone else to find. If a major piece of equipment breaks, tell the Laboratory Manager, the equipment monitor for the piece, or

P. I.(David Agard). UNDER NO CIRCUMSTANCE IS ANYONE TO LEAVE A PIECE IN NON WORKING CONDITION WITHOUT DEALING WITH IT.

3. When something goes wrong in the laboratory that is building related (i.e., sinks, windows, doors, floors) they can be handled by calling Capital Resources Management at 476-2021.

4. Anything you put into the sink to be cleaned, must have all tape & markings removed, be rinsed well with water, and placed into the tubs. Small thing such as bottle caps and spatulas are rinsed and placed into the plastic beakers marked caps and other small items. Sterilized water bottles are kept separately (out of the sink with other used glassware) being placed on the side counter of the back sink in S1076. Used autoclaved pipette canisters are placed on the side of the back sink in S1076. Any glassware which has seen ALP must be bleached overnight, rinsed and autoclaved.

5. Leave special media or solutions that need autoclaving on the glassware washer's cart (Emma's) with a note.

6. All orders are entered onto the lists at S1076 back bay's Desk. Please include vendor, quantity & item, catalog #, and price.

7. When orders arrive in the lab they are unpacked (empty boxes can be left outside the door, in the hall for disposal by the custodial crew). All packing slips are signed and dated by the unpacker, placed in the packing slip manila envelope on the freezer door (This includes all receipt slips from Bear Necessities & Tissue Culture Facility).

8. Chemical shelf and balances are to be kept clean and clear. Balance doors are to be kept closed.

9. Radioactive isotope usage and disposal must be entered in the big yellow Radiation Safety Log Book.

10. Solid Bacterial Wastes: Petri plates are disposed in the biohazard waste container pail, and the container top is placed on firmly. Do not overfill. Tie-up bag with autoclave tape and place bag on top of waste container to be autoclaved and disposed off. Replace red biohazard bag (double bag) in pail. Liquid cultures are first killed with a small amount bleach, letting sit for a few minutes and then rinsed down the drain with much tap water.

11. Recycle all foil. There are marked receptacles on the ledge next to all the sinks for foil recycling (Please do not overfill, a large bag of foil is located in S1076 middle bay)

12. No dry ice in sinks (cracks sink and drain), let it evaporate in the ice bucket after use. No agarose or acrylamide gels or solution containing such, are poured into the drains, it clogs them.

13. Common stocks and petri plates. Petri plates are poured by Marlene and are kept in the cold room, left middle bench. The common 1000X stocks of antibiotics, IPTG, trypsin are kept in the refrigerator freezer. Substrate stocks of 20mM (9.55mg/ml) p-Ala-Pro-Ala-pNa in 0.1M Tris pH8.0 are aloquoted on the S1076 freezer door.

14. Chemicals used in the lab are reordered when the supply gets low. Under no circumstance is anyone to leave an empty container on the chemical self. Chemicals are ordered when they are found in low supply, before we run out. You can request it to be ordered by noting it on the ordering list .

15. Always turn off equipment when finished (i.e., the spectrophotometer and pH meter).

16. Always lock the laboratory door when you are the last to leave, or when you leave the lab for any period of time during the evenings and weekend.

17. No food or drink is allowed in the lab and the walk-in cold room. There can't be any food containers or wrappers in any of the waste baskets in the lab.

18. Please limit your outgoing and incoming calls in the laboratory. Do not tie up the phone line with personal calls. Stopping work to answer the phone for others and taking messages, though courteous, is disruptive, time consuming, and sometimes an annoyance.

19. The S1076 microwave is not for heating food. Never melt agarose or solutions that contain ethidium bromide in the microwave.

20. Always use the hood for organic solvent and concentrated acids. Always use gloves, eye, and ear protection when necessary.