Description of the BUSTER manual
||© 1995-2008 by Global Phasing Limited
||All rights reserved.
This software is proprietary to and embodies the confidential
technology of Global Phasing Limited (GPhL). Possession, use,
duplication or dissemination of the software is authorised only
pursuant to a valid written licence from GPhL.
This manual describes how to use BUSTER, a
software system for crystallographic structure determination by
Bayesian statistical methods. It does not deal with more technical
tasks such as installing BUSTER on a
computer: for this, please refer to the Installation Guide (for Site
The manual consists of five chapters and four Appendices:
- Chapter 1 : Basic notions about BUSTER The purpose of BUSTER and the statistical principles it
- Chapter 2 :Present Capabilities of BUSTER This offers a survey of the types of
problems BUSTER can currently tackle and, for
each problem type, a description of the prerequisite information.
- Chapter 3 : The BUSTER Control Panel
and environment Explains how to deal with a browser as a Graphical
User Interface; explains what CCP4, the MTZ format and the TNT program are; and describes the first interface
pages you will have to deal with : the BUSTER
Control Panel (to access all the functionalities of BUSTER) and the Preferences page (to tailor certain
BUSTER and helper applications parameters to
your requirements and tastes).
- Chapter 4 : Preparing the BUSTER input The use of the graphical interface
is explained. Each field of the input form is described. Parameter
choices for typical problems are suggested.
- Chapter 5 : Browsing the BUSTER
output The use of the graphical interface for examining the
results produced by BUSTER is explained. The
main categories of results are described step by step.
- FAQ : Troubleshooting and FAQ Checks for possible
troubles are indicated, together with the actions to be taken.
- Appendix A : NCS in TNT A
simplified description of TNT
Non-Crystallographic Symmetry handling
- Appendix B : Real-space distributions in BUSTER A description of BUSTER algorithms for the generation of
low-resolution distributions to model missing atoms.
- Appendix C : TNT scripts used by
BUSTER A description of BUFFET scripts that run TNT programs during partial structure refinement.
Once you have browsed through the textual material contained in these
chapters, you are advised to browse the tutorials to learn by example.
BUSTER is a rapidly evolving program. Please
report all infelicities and bugs to the authors.
The writing of BUSTER was started in 1989 by
with subsequent contributions from
John Irwin (1994-1998),
Pietro Roversi (1995-2003),
Clemens Vonrhein (since 1998),
Eric Blanc (1998-2003),
Wlodek Paciorek (since 1998),
ClAuS Flensburg (since 2000) and
Oliver Smart (since 2004).
The graphical interface BUFFET was initially written by John Irwin
with contributions from all the other people mentioned above, plus
Eric de La Fortelle.
The use of BUSTER should be acknowledged in
all publications or reports mentioning results obtained by means of
it, and the following references should be cited:
Bricogne, G. (1993). Direct Phase Determination by Entropy
Maximisation and Likelihood Ranking: Status Report and
Perspectives. Acta Cryst. D49, 37-60.
Bricogne, G. (1997). The Bayesian Statistical Viewpoint on Structure
Determination: Basic Concepts and Examples, in Methods in
Enzymology, 276A, 361-423. C.W. Carter & R.M. Sweet,
Roversi, P., Blanc, E., Vonrhein, C., Evans, G. and Bricogne,
G. (2000). Modelling prior distributions of atoms for Macromolecular
Refinement and Completion. Acta Cryst., D56, 1313-1323.
Blanc, E., Roversi, P., Vonrhein, C., Flensburg, C., Lea, S. M. and
Bricogne, G. (2004). Refinement of severely incomplete structures with
maximum likelihood in BUSTER-TNT. Acta Cryst., D60,
Forthcoming publications on BUSTER will be
listed here in due course.
Last modification: 21.07.09