Stroud Lab
  updated 4/29/16

 

 

 

Featured Publications

Kapoor K, Finer-Moore J, Pedersen BP, Waight, Caboni L, Hillig R, Bringmann P, Heisler I, Mülle T, Siebeneicher H, Stroud RM (2016) Crystal structures of human GLUT1 bound to Cytochalasin B and novel inhibitors. Proc Natl Acad Sci USA. online Apr 12 2016 DOI 10.1073/ pnas.1603735113

Kintzer A, Stroud RM (2016) Structure, inhibition and regulation of two-pore channel TPC1 from Arabidopsis thaliana. Nature. 531: 258-62 See also SSRL news update; ALS news and update

Finer-Moore JS, Czudnochowski N, O'Connell JD IIIrd, Wang AL, Stroud RM (2015) Crystal Structure of the Human tRNA m(1)A58 Methyltransferase-tRNA3(Lys) Complex: Refolding of Substrate tRNA Allows Access to the Methylation Target. J Mol Biol 427: 3862-3876

Kumar H, Finer-Moore JS, Kaback HR, Stroud RM. Structure of LacY with an alpha-substituted galactoside: Connecting the binding site to the protonation site. (2015) Proc Natl Acad Sci USA. 2015;112(29):9004-9. doi: 10.1073/pnas.1509854112. PubMed PMID: 26157133; PMCID: PMC4517220.

Waight AB, Pedersen BP, Schlessinger A, Bonomi M, Chau BH, Roe-Zurz Z, Risenmay AJ, Sali A, Stroud RM (2013) Structural basis for alternating access of a eukaryotic calcium/proton exchanger. Nature. 499: 107-10. (PMCID: PMC3702627)
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Pedersen, B.P., Kumar, H., Waight, A.B., Risenmay, A., Roe-Zurz, Z., Chau, B., Schlessinger, A., Harries, W., Sali, A., Johri, A.K., Stroud, R.M. (2013). Crystal structure of a eukaryoticphosphate transporter.  Nature. (PMID: 23542591)
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Pieper, U., Schlessinger, A., Kloppmann, E., Chang, G.A., Chou, J.J., Dumont, M.E., Fox, B.G., Fromme, P., Hendrickson, W.A., Malkowski, M.G., Rees, D.C., Stokes, D.L., Stowell, M.H., Wiener, M.C., Rost, B., Stroud, R.M., Stevens, R.C., Sali, A. (2013). Coordinating the impact of structural genomics on the human α-helical transmembrane proteome. Nat Struct Mol Biol 20: 135-8. (PMID: 23381628/PMCID: in process).
[This Commentary by 9 membrane centers received 1,327 visits and 2,233 page views since it was published in February 2013. It was ranked #4 in article requests for the month of February.]
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Chaudhary, S., Pak, J.E., Gruswitz, F., Sharma, V., Stroud, R.M. (2012). Overexpressing Human Membrane Proteins in Stably Transfected and Clonal Human Embryonic Kidney 293S Cells. Nature Protocols
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Latest News

Filoviruses, such as Ebola virus require host-cell receptors, endocytosis, proteolytic cleavage, and fusion with the endolysosomal membrane for release of viral material into the cytoplasm. Two-pore channels (TPCs) comprise a subfamily (TPC1-3) of eukaryotic voltage- and ligand-gated cation channels that contain two non-equivalent tandem pore-forming subunits that then dimerize to form quasi-tetramers and orchestrate the trafficking of Filoviruses, including Ebola, in human cells.
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Kintzer A, Stroud RM (2016) Structure, inhibition and regulation of two-pore channel TPC1 from Arabidopsis thaliana. Nature. 531: 258-62

New research reveals the mechanism behind a cancer-relevant inhibition of human sugar transporting protein. The hope is that this will guide future drug design targeting sugar uptake mechanisms. This will ultimately lead to progress in a number of important common conditions, such as diabetes and cancer.

Our group revealed how different inhibitors of the human sugar transporter GLUT1 (figure) bind to a central cavity in the protein to inhibit its function. The results show a surprisingly promiscuous internal binding pocket with submicromolar affinities for chemically distinct substrates. With these results, the hope is that this will guide future drug design targeting sugar uptake mechanisms.

Tumors and cancer cells are highly dependent on sugar uptake to maintain their rapid growth rate. It has been proposed that a regulation or reduction of cellular sugar uptake might have therapeutic benefits. While not being a panacea, a reduction in cancer growth rate could allow other treatments time to reach full effect, and potentially could allow a much improved efficacy of other chemotherapeutics.

Kapoor K, Finer-Moore J, Pedersen BP, Waight, Caboni L, Hillig R, Bringmann P, Heisler I, Mülle T, Siebeneicher H, Stroud RM (2016) Crystal structures of human GLUT1 bound to Cytochalasin B and novel inhibitors Proc Natl Acad Sci USA. online Apr 12 2016 DOI 10.1073/pnas.1603735113

Waight AB, Pedersen BP, Schlessinger A, Bonomi M, Chau BH, Roe-Zurz Z, Risenmay AJ, Sali A, Stroud RM (2013) Structural basis for alternating access of a eukaryotic calcium/proton exchanger. Nature. 499: 107-10. (PMCID: PMC3702627)
Download (PDF) »

This Commentary by 9 membrane centers received 1,327 visits and 2,233 page views since it was published in February 2013. It was ranked #4 in article requests for the month of February.
Download (PDF) »

Pedersen, B.P., Kumar, H., Waight, A.B., Risenmay, A., Roe-Zurz, Z., Chau, B., Schlessinger, A., Harries, W., Sali, A., Johri, A.K., Stroud, R.M. (2013). Crystal structure of a eukaryoticphosphate transporter.  Nature. (PMID: 23542591)
Download (PDF) »

Dr. Stroud's laboratory has been selected by the Guthy-Jackson Charitable Foundation (GJCF) to supply full length human AQP4 for all of their associated neuromyelitis optica (NMO) research laboratories. Links to the GJCF and the NMO community are below. http://www.guthyjacksonfoundation.org/ http://spectrum.guthyjacksonfoundation.org/
[Sept 2010]

Biophysical Society announces Dr. Stroud as the recipient of the 2009 Anatrace Membrane Protein Award.
[Sept 2008]

Dr. Stroud receives the 2008 Hans Neurath Award. [July 2008]
View article at Proteinsociety.org »

Robert Stroud profiled in PNAS.
[April 4, 2006]
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