Current projects focus on ion channels, transporters, and combating antibodies in autoimmune disease. Transporters include those involved in drug resistance, and in packaging neurotransmitters. Infectious disease focus areas include malaria, mycobacterium tuberculosis and HIV.
Filoviruses, such as Ebola virus require host-cell receptors, endocytosis, proteolytic cleavage, and fusion with the endolysosomal membrane for release of viral material into the cytoplasm. Two-pore channels (TPCs) comprise a subfamily (TPC1-3) of eukaryotic voltage- and ligand-gated cation channels that contain two non-equivalent tandem pore-forming subunits that then dimerize to form quasi-tetramers and orchestrate the trafficking of Filoviruses, including Ebola, in human cells.
New research reveals the mechanism behind a cancer-relevant inhibition of human sugar transporting protein. The hope is that this will guide future drug design targeting sugar uptake mechanisms. This will ultimately lead to progress in a number of important common conditions, such as diabetes and cancer.
Kapoor K, Finer-Moore J, Pedersen BP, Waight, Caboni L, Hillig R, Bringmann P, Heisler I, Mülle T, Siebeneicher H, Stroud RM (2016) Crystal structures of human GLUT1 bound to Cytochalasin B and novel inhibitors. Proc Natl Acad Sci USA. online Apr 12 2016 DOI 10.1073/ pnas.1603735113
Kintzer A, Stroud RM (2016) Structure, inhibition and regulation of two-pore channel TPC1 from Arabidopsis thaliana. Nature. 531: 258-62 See also SSRL news update; ALS news and update
Finer-Moore JS, Czudnochowski N, O'Connell JD IIIrd, Wang AL, Stroud RM (2015) Crystal Structure of the Human tRNA m(1)A58 Methyltransferase-tRNA3(Lys) Complex: Refolding of Substrate tRNA Allows Access to the Methylation Target. J Mol Biol 427: 3862-3876