2004–Current

2009

Catalytically-active complex of HIV-1 integrase with a viral DNA substrate binds anti-integrase drugs. Akram Alian, Sarah L. Griner, Vicki Chiang, Manuel Tsiang, Gregg Jones, Gabriel Birkus, Romas Geleziunas, Andrew D. Leavitt, and Robert M. Stroud
PNAS, (May 2009); 106: 8192-8197. Download (PDF)

Crystal structure of human aquaporin 4 at 1.8 Å and its mechanism of conductance. Joseph D. Ho, Ronald Yeh, Andrew Sandstrom, Ilya Chorny, William E. C. Harries, Rebecca A. Robbins, Larry J. W. Miercke, and Robert M. Stroud PNAS, (May 2009); 106: 7437-7442. Download (PDF)

The unfolded protein response signals through high-order assembly of Ire1. Alexei V. Korennykh, Pascal F. Egea, Andrei A. Korostelev, Janet Finer-Moore, Chao Zhang, Kevan M. Shokat, Robert M. Stroud and Peter Walter. (2009) Nature 457, 687-93.

Selecting optimum eukaryotic integral membrane proteins for structure determination by rapid expression and solubilization screening. Min Li, Frank A Hays, Zygy Roe-Zurz, Linda Vuong, Libusha Kelly, Chi Min Ho, Rebecca M Robbins, Ursula Pieper, Joseph O'Connell III, Larry J Miercke, Kathleen M Giacomini, Andrej Sali, and Robert M Stroud. (2009) J Mol Biol 385(3); 820-30.

2008

Ratiocinative screen of eukaryotic integral membrane protein expression and solubilization for structure determination. Franklin A Hays, Zygy Roe-Zurz, Min Li, Libusha Kelly, Franz Gruswitz, Andrej Sali, and Robert M Stroud. (2008) J Struct Funct Genomics.

Crystal structure of bovine mitochondrial factor B at 0.96-Å resolution. John K. Lee, Grigory I. Belogrudov, and Robert M. Stroud (2008) PNAS 105; 13379-84.
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Crystal structure of the aquaglyceroporin PfAQP from the malarial parasite Plasmodium falciparum. Zach E. Newby, Josepeh O'Connell III, Yaneth Robles-Colmenares, Shahram Khademi, Larry J. Miercke, and Robert M. Stroud. (2008) Nat Struct Mol Biol 15(6); 619-25.
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Structure of a TrmA–RNA complex: A consensus RNA fold contributes to substrate selectivity and catalysis in m5U methyltransferases. Akram Alian, Tom T. Lee, Sarah L. Griner, Robert M. Stroud, and Janet Finer-Moore. (2008) PNAS 105; 6876-6881.
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Structural and biochemical insights into the dicing mechanism of mouse Dicer: A conserved lysine is critical for dsRNA cleavage. Zhihua Du, John K. Lee, Richard Tjhen, Robert M. Stroud, and Thomas L. James. (2008) PNAS 105; 2391-2396.
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2007

How U38, 39, and 40 of many tRNAs become the targets for pseudouridylation by TruA. Mol Cell 26: 189-203. Hur, S., Stroud, R.M. (2007).

TARP auxiliary subunits switch AMPA receptor antagonists into partial agonists. Science 318: 815-817. Menuz, K., Stroud, R.M., Nicoll, R.A., Hays, F.A. (2007).

The crystal structure of E. coli rRNA pseudouridine synthase RluE. J Mol Biol 367: 1459-1470252. Pan, H., Ho, J.D., Stroud, R.M., Finer-Moore, J. (2007).

X-ray structures of the signal recognition particle receptor reveal targeting cycle intermediates. PLoS ONE 2: e607. Reyes, C.L., Rutenber, E., Walter, P., Stroud, R.M. (2007).

Cell-free complements in vivo expression of the E. coli membrane proteome. Protein Sci 16: 966-976. Savage, D.F., Anderson, C.L., Robles-Colmenares, Y., Newby, Z.E., Stroud, R.M. (2007).

Structural basis of aquaporin inhibition by mercury. J Mol Biol 368: 607-617. Savage, D.F., Stroud, R.M. (2007).

Transmembrane transporters: an open and closed case. Proc Natl Acad Sci U S A 104: 1445-1446.Stroud, R.M. (2007).

Inhibitory complex of the transmembrane ammonia channel, AmtB, and the cytosolic regulatory protein, GlnK, at 1.96Å. Franz Gruswitz, Joseph O'Connell, III, and Robert M. Stroud. (2007) PNAS 104; 42-47.
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2006

The Amt/MEP/Rh family: structure of AmtB and the mechanism of ammonia gas conduction. Khademi, S., and Stroud, R.M. (2006) Physiology (Bethesda)21: 419-429.

Gas Channels for Ammonia. Khademi, S., and Stroud,R.M. (2006), Invited Chapter in Royal Society of Chemistry: Structural Biology of Membrane Proteins, ed., Reinhard Grisshammer and Susan K. Buchanan. Chapter 12, 212-234.

Aquaporins: Integral Membrane Channel Proteins. Stroud, R.M., Harries, W.E.C., Lee, J, Khademi, S., and Savage, D (2006), Invited Chapter  in Royal Society of Chemistry: Structural Biology of Membrane Proteins, ed., Reinhard Grisshammer and Susan K. Buchanan. Chapter 11, 195-211.

The Role of Protein Dynamics in Thymidylate Synthase Catalysis: Variants of Conserved 2'-Deoxyuridine 5'-Monophosphate (dUMP)-Binding Tyr-261. Zachary Newby, Tom T. Lee, Richard J. Morse, Yaoquan Liu, Lu Liu, Prasanna Venkatraman, Daniel V. Santi, Janet S. Finer-Moore, and Robert M. Stroud. (2006) Biochemistry Vol 45, 24.
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Substrate Recognition by RNA 5-Methyluridine Methyltransferases and Pseudouridine Synthases: A Structural Perspective. Sun Hur, Robert M. Stroud, and Janet Finer-Moore. (2006) The Journal of Biological Chemistry Vol. 281, No. 51, pp. 38969–38973.
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The Structure of a Ketoreductase Determines the Organization of the b-Carbon Processing Enzymes of Modular Polyketide Synthases. Adrian T. Keatinge-Clay, and Robert M. Stroud. (2006) Structure 14, 737–748.
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2005

Structural Basis for Conductance by the Archaeal Aquaporin AqpM at 1.68Å. Lee, J.K., Stroud, R.M.  (2005) Proc. Nat Acad Sci U S A 102, 18932-7.

On the Mechanism of Sensing Unfolded Protein in the Endoplasmic Reticulum.Credle, J.J., Finer-Moore, J.S., Papa, F.R., Stroud, R.M. and Walter, P. (2005) Proc Nat Acad Sci  102, 18773-18784

A unique RNA fold in the rumA-RNA-cofactor ternary complex contributes to substrate selectivity and enzymatic function. Lee, T., Agarwalla, S., Stroud, R.M. (2005) Cell 120, 599-611.
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Targeting proteins to membranes: structure of the signal recognition particle. Egea, P.F., Stroud, R.M., Walter, P. (2005) Current Opinion in Structural Biology 15:213-220.
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2004

De novo design of an IL-4 antagonist and its structure at 1.9Å. LaPorte, S.L., Forsyth, C.M., Cunningham, B.C., Miercke, L.J., Akhaven, D., Stroud, R.M. (2004) PNAS vol 102 no.6 1889-1894.
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Mechanism of association and reciprocal activation of two GTPases.
Shan, S. O., Stroud, R. M., Walter, P. (2004) PLoS Biol 2, e320
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An antibiotic factory caught in action.
Keatinge-Clay, A. T., Maltby, D. A., Medzihradszky, K. F., Khosla, C., Stroud, R. M. (2004) Nat Struct Mol Biol 11, 888-893.
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Mechanism of Ammonia Transport by Amt/MEP/Rh: Structure of AmtB at 1.35Å.
Khademi, S., O'Connell III, J., Remis, J., Robles-Colmenares, Y., Miercke L.J.W., Stroud, R.M. (2004) Science 305, 1587-1594. (Cover Article with Perspective by Knepper and Agre, 1573-1574)
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The Channel Architecture of Aquaporin 0 At 2.2 Å Resolution.
Harries, W.E.C., Akhavan, D., Miercke, L.J.W., Khademi, S., Stroud, R.M. (2004) Proc Nat Acad Sci 101, 14045-14050.
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Unraveling the interface of signal recognition particle and its receptor using chemical cross-linking and tandem mass spectrometry.
Chu, F., Shan, S.O. Moustakas, D.T., Alber, F., Egea, P.F., Stroud, R.M., Walter, P. Alma L. Burlingame, A.L. (2004) Proc. Nat Acad Sci 101, 16454-9
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Water and glycerol permeation through the glycerol channel GlpF and the aquaporin family.
Lee J.K., Khademi S., Harries W., Savage D., Miercke L., Stroud R.M. (2004). J Synchrotron Radiat 11, 86.

Mechanistic diversity of cytokine receptor signaling across cell membranes. Stroud R.M., Wells J.A. (2004). Science STKE 2004, re7.
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Substrate twinning activates the signal recognition particle and its receptor. Egea, P.F., Shan, S.O., Napetschnig, J., Savage, D.F., Walter, P., and Stroud, R.M. (2004) Nature 426 215-221.
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Redox reactions of the iron-sulfur cluster in a ribosomal RNA methyltransferase, RumA: optical and EPR studies. Agarwalla, S., Stroud, RM., Gaffney B.J. (2004). J Biol Chem 279, 34123-34129.
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